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Reduced SDS-PAGE analysis of purified isotype control antibodies. M) Mouse IgG2a, NullFc™, H) Human IgG1 NullFc™.
Flow cytometry of human peripheral blood lymphocytes stained isotype control rabbit IgG followed by Alexa Fluor 647-anti-rabbit IgG or just secondary alone. A) SSC vs. Secondary alone B) SSC vs Isotype control + secondary
Flow cytometry of human peripheral blood lymphocytes stained with isotype control rabbit IgG followed by Alexa Fluor 647-anti-rabbit IgG. A) CD4 vs. isotype control, B) CD19 vs isotype control.
C0108 Image 04
Recombinant Isotype Controls
These recombinant isotype controls are made with the same molecular formats and production methods as our other recombinant antibodies making them ideal controls for your experiments.
Catalog Items:
- #202101 – Rabbit IgG, kappa – 50 µg – $79
- #202102 – Mouse IgG2a, kappa NullFc™ – 50 µg – $79
- #202103 – Human IgG1, kappa NullFc™ – 50 µg – $79
- #202104 – Biotinylated Rabbit Fab – 50 µg – $99
$79.00 – $99.00
Specifications
| Clone | C0108 |
| Discovery Method | Rabbit germline IgG genes were used for the majority of the variable domains. The 2 CDR3 domains were taken from 2 different antibodies, with several point mutations made to critical residues to produce a non-functional antibody. Tested and shown to be non-reactive to human and rabbit blood cells, and human and bovine serum proteins |
| Available Formats | Rabbit IgG/kappa; Mouse IgG2a/kappa with NullFc™ (compatible with traditional anti-mouse secondary reagents); Human IgG1, kappa with NullFc™ (compatible with traditional anti-human secondary reagents); Biotinylated rabbit Fab with a single defined biotin and His-tag at the C-terminus of the heavy chain |
| Host Species | Rabbit |
| Immunogen | Not applicable |
| Verified Reactivity | None |
| Verified Applications | Multiple |
| Concentration | 0.5 mg/ml |
| Production | Transient transfection expression system in mammalian HEK293 cells |
| Purification | Protein A affinity chromatography (MAb), Immobilized metal ion chromatography (Fab) |
| Formulation | PBS pH 7.2 with 0.08% sodium azide |
| Storage | 4° C for up to 3 months, for longer storage aliquot and store at -20° C |
| Target | None |
| Alternate Names | Not applicable |
| Target Description | Not applicable |
| Uniprot | Not applicable |