Anti-human CD27 Recombinant Antibody

This anti-human CD27 recombinant antibody is available in multiple formats for flexibility. Mouse NullFc™ version has greatly reduced Fc receptor binding for cleaner results, yet is still compatible with traditional secondary reagents. Verified to work for non-human primate CD27 as well.

Catalog Items:

  • # 211301 – Rabbit IgG, kappa  – 100 µg – $49
  • # 211302 – Mouse IgG2a, kappa NullFc™ – 100 µg – $49
  • # 211303 – Biotinylated rabbit Fab – 100 µg – $69

$49.00$69.00

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SKU N/A Category

Specifications

Clone AB27H06
Discovery Method Rabbits were immunized with HEK293 cells expressing human CD27, and antigen positive B cells were single cell sorted. The variable domain sequences were cloned, sequenced and subcloned into expression vectors containing the various species and isotype constant domains
Available Formats Rabbit IgG/kappa; Mouse IgG2a/kappa with NullFc™ (compatible with traditional anti-mouse secondary reagents); Biotinylated rabbit Fab with a single defined biotin and His-tag at the C-terminus of the heavy chain
Host Species Rabbit
Immunogen Human CD27 (accession NP_001233.2) recombinantly expressed on HEK293 cells
Verified Reactivity Human, cynomolgus monkey (Macaca fascicularis)
Verified Applications Flow Cytometry
Concentration 0.5 mg/ml
Production Transient transfection expression system in mammalian HEK293 cells
Purification Protein A affinity chromatography (MAb), Immobilized metal ion chromatography (Fab)
Formulation PBS pH 7.2 with 0.08% sodium azide
Storage 4° C for up to 3 months, for longer storage aliquot and store at -20° C
Target CD27
Alternate Names TNFRSF7, T14, S152
Target Description CD27 is a member of the TNF receptor superfamily. This co-stimulatory molecule is expressed on many peripheral blood monocytes including subsets of T, B and NK cells. CD27 binds to CD70 and may play a role in T cell activation and survival.
Uniprot P26842

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