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Flow cytometry of human peripheral blood lymphocytes stained with anti-human CD27 rabbit IgG followed by Alexa Fluor 647-anti-rabbit IgG. A) CD4 vs. CD27. B) CD19 vs CD27.
Flow cytometry of human peripheral blood lymphocytes stained with isotype control rabbit IgG followed by Alexa Fluor 647-anti-rabbit IgG. A) CD4 vs. isotype control. B) CD19 vs isotype control.
HEK293 cells were transfected with either human or cynomolgus CD27 or no DNA (Mock) and stained with anti-human CD27 rabbit IgG followed by Alexa Fluor 647-anti-rabbit IgG.
Anti-human CD27 Recombinant Antibody
This anti-human CD27 recombinant antibody is available in multiple formats for flexibility. Mouse NullFc™ version has greatly reduced Fc receptor binding for cleaner results, yet is still compatible with traditional secondary reagents. Verified to work for non-human primate CD27 as well.
Catalog Items:
- # 211301 – Rabbit IgG, kappa – 100 µg – $49
- # 211302 – Mouse IgG2a, kappa NullFc™ – 100 µg – $49
- # 211303 – Biotinylated rabbit Fab – 100 µg – $69
Terms
The buyer of this product is prohibited from using, studying, sequencing or analyzing a product or any portion thereof to learn the respective construction, sequence, composition, design, operation or to produce a copy, reverse engineer or produce an improved version of the product or a functionally similar material. If you do not accept these terms, please contact us at info@cobaltbiologics.com to arrange for a return and full refund of un-opened product.
$49.00 – $69.00
Specifications
| Clone | AB27H06 |
| Discovery Method | Rabbits were immunized with HEK293 cells expressing human CD27, and antigen positive B cells were single cell sorted. The variable domain sequences were cloned, sequenced and subcloned into expression vectors containing the various species and isotype constant domains |
| Available Formats | Rabbit IgG/kappa; Mouse IgG2a/kappa with NullFc™ (compatible with traditional anti-mouse secondary reagents); Biotinylated rabbit Fab with a single defined biotin and His-tag at the C-terminus of the heavy chain |
| Host Species | Rabbit |
| Immunogen | Human CD27 (accession NP_001233.2) recombinantly expressed on HEK293 cells |
| Verified Reactivity | Human, cynomolgus monkey (Macaca fascicularis) |
| Verified Applications | Flow Cytometry |
| Concentration | 0.5 mg/ml |
| Production | Transient transfection expression system in mammalian HEK293 cells |
| Purification | Protein A affinity chromatography (MAb), Immobilized metal ion chromatography (Fab) |
| Formulation | PBS pH 7.2 with 0.08% sodium azide |
| Storage | 4° C for up to 3 months, for longer storage aliquot and store at -20° C |
| Target | CD27 |
| Alternate Names | TNFRSF7, T14, S152 |
| Target Description | CD27 is a member of the TNF receptor superfamily. This co-stimulatory molecule is expressed on many peripheral blood monocytes including subsets of T, B and NK cells. CD27 binds to CD70 and may play a role in T cell activation and survival. |
| Uniprot | P26842 |