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Flow cytometry of human peripheral blood lymphocytes stained with anti-human CD3 rabbit IgG followed by Alexa Fluor 647-anti-rabbit IgG. A) CD4 vs. CD3. B) CD19 vs CD3.
Flow cytometry of human peripheral blood lymphocytes stained with isotype control rabbit IgG followed by Alexa Fluor 647-anti-rabbit IgG. A) CD4 vs. isotype control. B) CD19 vs isotype control.
HEK293 cells were transfected with either cynomolgus or mouse CD3epsilon or no DNA (Mock) and stained with anti-human CD3 rabbit IgG followed by Alexa Fluor 647-anti-rabbit IgG.
Anti-human CD3 Recombinant Antibody
This recombinant antibody binds to human and non-human primate CD3 (epsilon subunit), which is expressed on T cells. Available in 3 formats for added flexibility.
Catalog Items:
- # 201701 – Rabbit IgG, kappa – 50 µg – $89
- # 201702 – Mouse IgG2a, kappa NullFc™ – 50 µg – $89
- # 201703 – Biotinylated rabbit Fab – 50 µg – $129
Terms
The buyer of this product is prohibited from using, studying, sequencing or analyzing a product or any portion thereof to learn the respective construction, sequence, composition, design, operation or to produce a copy, reverse engineer or produce an improved version of the product or a functionally similar material. If you do not accept these terms, please contact us at info@cobaltbiologics.com to arrange for a return and full refund of un-opened product.
$89.00 – $129.00
Specifications
| Clone | 283F7 |
| Discovery Method | Rabbits were immunized with human Jurkat cells that naturally express CD3, and antigen-positive B cells were single-cell sorted. The variable domain sequences were cloned, sequenced, and subcloned into expression vectors containing the various species and isotype constant domains |
| Available Formats | Rabbit IgG/kappa; Mouse IgG2a/kappa with NullFc™ (compatible with traditional anti-mouse secondary reagents); Biotinylated rabbit Fab with a single defined biotin and His-tag at the C-terminus of the heavy chain |
| Host Species | Rabbit |
| Immunogen | Jurkat human cell line |
| Verified Reactivity | Human, cynomolgus monkey (Macaca fascicularis), NOT mouse |
| Verified Applications | Flow Cytometry |
| Concentration | 0.5 mg/ml |
| Production | Transient transfection expression system in mammalian HEK293 cells |
| Purification | Protein A affinity chromatography (MAb), Immobilized metal ion chromatography (Fab) |
| Formulation | PBS pH 7.2 with 0.08% sodium azide |
| Storage | 4° C for up to 3 months, for longer storage aliquot and store at -20° C |
| Target | CD3 epsilon |
| Alternate Names | CD3e, T3 |
| Target Description | CD3 epsilon is part of the T-cell receptor (TCR)/CD3 complex. When the TCR is activated by an antigen-presenting cell, the signal is transmitted across the cell membrane by the CD3 chains. CD3 is considered a pan-T cell marker. |
| Uniprot | P07766 |