Anti-human CD3 Recombinant Antibody

This recombinant antibody binds to human and non-human primate CD3 (epsilon subunit), which is expressed on T cells. Available in 3 formats for added flexibility.

Catalog Items:

  • # 201701 – Rabbit IgG, kappa – 50 µg – $89
  • # 201702 – Mouse IgG2a, kappa NullFc™ – 50 µg – $89
  • # 201703 – Biotinylated rabbit Fab – 50 µg – $129

$89.00$129.00

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SKU N/A Category

Specifications

Clone 283F7
Discovery Method Rabbits were immunized with human Jurkat cells that naturally express CD3, and antigen-positive B cells were single-cell sorted. The variable domain sequences were cloned, sequenced, and subcloned into expression vectors containing the various species and isotype constant domains
Available Formats Rabbit IgG/kappa; Mouse IgG2a/kappa with NullFc™ (compatible with traditional anti-mouse secondary reagents); Biotinylated rabbit Fab with a single defined biotin and His-tag at the C-terminus of the heavy chain
Host Species Rabbit
Immunogen Jurkat human cell line
Verified Reactivity Human, cynomolgus monkey (Macaca fascicularis), NOT mouse
Verified Applications Flow Cytometry
Concentration 0.5 mg/ml
Production Transient transfection expression system in mammalian HEK293 cells
Purification Protein A affinity chromatography (MAb), Immobilized metal ion chromatography (Fab)
Formulation PBS pH 7.2 with 0.08% sodium azide
Storage 4° C for up to 3 months, for longer storage aliquot and store at -20° C
Target CD3 epsilon
Alternate Names CD3e, T3
Target Description CD3 epsilon is part of the T-cell receptor (TCR)/CD3 complex. When the TCR is activated by an antigen-presenting cell, the signal is transmitted across the cell membrane by the CD3 chains. CD3 is considered a pan-T cell marker.
Uniprot P07766

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