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HEK293 cells were transfected with either human or cynomolgus CD37 or no DNA (Mock) and stained with anti-human CD37 rabbit IgG followed by Alexa Fluor 647-anti-rabbit IgG.
Flow cytometry of human peripheral blood lymphocytes stained with anti-human CD37 rabbit IgG followed by Alexa Fluor 647-anti-rabbit IgG. A) CD4 vs. CD37. B) CD19 vs CD37.
Flow cytometry of human peripheral blood lymphocytes stained with isotype control rabbit IgG followed by Alexa Fluor 647-anti-rabbit IgG. A) CD4 vs. isotype control. B) CD19 vs isotype control.
Reduced and non-reduced SDS-PAGE analysis of purified lots of recombinant anti-human CD37 antibodies.
XA1531 Image 05
Anti-human CD37 Recombinant Antibody
Human CD37 antibody that is potent and highly specific. Binds strongly to CD19+ positive B cells and weakly but significantly to some T cells and myeloid cells. Verified to work in non-human primate CD37 as well.
Catalog Items:
- # 201401 – Rabbit IgG, kappa – 50 µg – $79
- # 201402 – Mouse IgG2a, kappa NullFc™ – 50 µg – $79
- # 201402 – Biotinylated rabbit Fab – 50 µg – $99
Terms
The buyer of this product is prohibited from using, studying, sequencing or analyzing a product or any portion thereof to learn the respective construction, sequence, composition, design, operation or to produce a copy, reverse engineer or produce an improved version of the product or a functionally similar material. If you do not accept these terms, please contact us at info@cobaltbiologics.com to arrange for a return and full refund of un-opened product.
$79.00 – $99.00
Specifications
| Clone | XA1531 |
| Discovery Method | Rabbits were immunized with HEK293 cells expressing human CD37, and antigen-positive B cells were single-cell sorted. The variable domain sequences were cloned, sequenced, and subcloned into expression vectors containing the various species and isotype constant domains |
| Available Formats | Rabbit IgG/kappa; Mouse IgG2a/kappa with NullFc™ (compatible with traditional anti-mouse secondary reagents); Biotinylated rabbit Fab with a single defined biotin and His-tag at the C-terminus of the heavy chain |
| Host Species | Rabbit |
| Immunogen | Human CD37 (accession NM_001774) recombinantly expressed on HEK293 cells |
| Verified Reactivity | Human, cynomolgus monkey (Macaca fascicularis) |
| Verified Applications | Flow Cytometry |
| Concentration | 0.5 mg/ml |
| Production | Transient transfection expression system in mammalian HEK293 cells |
| Purification | Protein A affinity chromatography (MAb), Immobilized metal ion chromatography (Fab) |
| Formulation | PBS pH 7.2 with 0.08% sodium azide |
| Storage | 4° C for up to 3 months, for longer storage aliquot and store at -20° C |
| Target | CD37 |
| Alternate Names | GP52-40, TSPAN26 |
| Target Description | CD37 is a type II transmembrane protein in the tetraspanin family. CD37 associates with integrins and other transmembrane 4 family members. CD37 is expressed highly in B cells and also at a low level in myeloid cells and T cells. |
| Uniprot | P11049 |