Flow cytometry of human peripheral blood lymphocytes stained with anti-human CD79b rabbit IgG followed by Alexa Fluor 647-anti-rabbit IgG. A) CD4 vs. CD79b. B) CD19 vs CD79b.
Flow cytometry of human peripheral blood lymphocytes stained with isotype control rabbit IgG followed by Alexa Fluor 647-anti-rabbit IgG. A) CD4 vs. isotype control. B) CD19 vs isotype control.
HEK293 cells were transfected with either human, mouse, cynomolgus CD79b or no DNA (Mock) and stained with anti-human CD79b rabbit IgG followed by Alexa Fluor 647-anti-rabbit IgG.
Anti-human CD79b Recombinant Antibody
This recombinant antibody binds strongly to CD79b which is part of the B cell receptor complex (BCR), thus making it a very useful B cell marker antibody. Available in 3 formats for added flexibility.
Catalog Items:
- # 201601 – Rabbit IgG, kappa – 50 µg – $89
- # 201602 – Mouse IgG2a, kappa NullFc™ – 50 µg – $89
- # 201603 – Biotinylated rabbit Fab – 50 µg – $129
- # 201601-2MG – Rabbit IgG, kappa – 2 mg – $2,000
- # 201602-2MG – Mouse IgG2a, kappa NullFc™ – 2 mg – $2,000
$89.00 – $2,000.00
Specifications
| Clone | XP2238 |
| Discovery Method | Rabbits were immunized with HEK293 cells expressing human CD79b, and antigen-positive B cells were single-cell sorted. The variable domain sequences were cloned, sequenced, and subcloned into expression vectors containing the various species and isotype constant domains |
| Available Formats | Rabbit IgG/kappa; Mouse IgG2a/kappa with NullFc™ (compatible with traditional anti-mouse secondary reagents); Biotinylated rabbit Fab with a single defined biotin and His-tag at the C-terminus of the heavy chain |
| Host Species | Rabbit |
| Immunogen | Human CD79b (accession NM_000626) recombinantly expressed on HEK293 cells |
| Verified Reactivity | Human, cynomolgus monkey (Macaca fascicularis) |
| Verified Applications | Flow Cytometry |
| Concentration | 0.5 – 1.0 mg/ml |
| Production | Transient transfection expression system in mammalian HEK293 cells |
| Purification | Protein A affinity chromatography (MAb), Immobilized metal ion chromatography (Fab) |
| Formulation | PBS pH 7.2 with 0.08% sodium azide |
| Storage | 4° C for up to 3 months, for longer storage aliquot and store at -20° C |
| Target | CD79b |
| Alternate Names | B29, AGM6, IGB |
| Target Description | CD79b heterodimerizes with CD79a to become part of the B cell receptor complex (BCR). A type I transmembrane protein of 37-39 kD, CD79b is expressed on B cells that express surface Ig. |
| Uniprot | P40259 |
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