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Flow cytometry of human peripheral blood lymphocytes stained with anti-human TIGIT rabbit IgG followed by Alexa Fluor 647-anti-rabbit IgG. A) CD4 vs. TIGIT. B) CD19 vs TIGIT.
Flow cytometry of human peripheral blood lymphocytes stained with isotype control rabbit IgG followed by Alexa Fluor 647-anti-rabbit IgG. A) CD4 vs. isotype control. B) CD19 vs isotype control.
HEK293 cells were transfected with either human, cynomolgus, or mouse TIGIT or no DNA (Mock) and stained with anti-human TIGIT human IgG NullFc followed by PE-anti-human IgG.
Reduced (R) and non-reduced (NR) SDS-PAGE analysis of purified recombinant anti-human TIGIT antibody.
TG12-1 Image 05
Anti-human TIGIT Recombinant Antibody
This TIGIT recombinant antibody is cross-reactive with human, non-human primate, and mouse TIGIT. So you can use the same antibody in both your mouse and human studies.
Catalog Items:
- #201501 – Rabbit IgG, kappa – 50 µg – $49
- #201503 – Mouse IgG2a, kappa NullFc™ – 50 µg – $49
- #201502 – Biotinylated Rabbit Fab – 50 µg – $79
$49.00 – $79.00
Specifications
| Clone | TG12-1 |
| Discovery Method | Rabbits were immunized with HEK293 cells expressing human TIGIT, and antigen-positive B cells were single-cell sorted. The variable domain sequences were cloned, sequenced, and subcloned into expression vectors containing the various species and isotype constant domains |
| Available Formats | Rabbit IgG/kappa; Mouse IgG2a/kappa with NullFc™ (compatible with traditional anti-mouse secondary reagents); Biotinylated rabbit Fab with a single defined biotin and His-tag at the C-terminus of the heavy chain |
| Host Species | Rabbit |
| Immunogen | Human TIGIT (accession NM_173799) recombinantly expressed on HEK293 cells |
| Verified Reactivity | Human, cynomolgus monkey (Macaca fascicularis), mouse |
| Verified Applications | Flow Cytometry |
| Concentration | 0.5 mg/ml |
| Production | Transient transfection expression system in mammalian HEK293 cells |
| Purification | Protein A affinity chromatography (MAb), Immobilized metal ion chromatography (Fab) |
| Formulation | PBS pH 7.2 with 0.08% sodium azide |
| Storage | 4° C for up to 3 months, for longer storage aliquot and store at -20° C |
| Target | TIGIT |
| Alternate Names | VSIG9, VSTM3 |
| Target Description | TIGIT (T-cell immunoreceptor with immunoglobulin and ITIM domains) is expressed on a subset of T cells (cytotoxic, memory and Tregs as well as NK cells. TIGIT binds to CD155 (PVR) and with lower affinity to CD112 (PVRL2). TIGIT binding results in increased secretion of IL10 and decreased secretion of IL12B and also causes the suppression of T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. |
| Uniprot | Q495A1 |
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Terms
The buyer of this product is prohibited from using, studying, sequencing or analyzing a product or any portion thereof to learn the respective construction, sequence, composition, design, operation or to produce a copy, reverse engineer or produce an improved version of the product or a functionally similar material. If you do not accept these terms, please contact us at info@cobaltbiologics.com to arrange for a return and full refund of un-opened product.