Flow cytometry of human peripheral blood lymphocytes stained with anti-human CD112R rabbit IgG followed by Alexa Fluor 647-anti-rabbit IgG. A) CD4 vs. CD112R. B) CD19 vs CD112R.
Flow cytometry of human peripheral blood lymphocytes stained with isotype control rabbit IgG followed by Alexa Fluor 647-anti-rabbit IgG. A) CD4 vs. isotype control. B) CD19 vs isotype control.
HEK293 cells were transfected with either human or cynomolgus CD112R or no DNA (Mock) and stained with anti-human CD112R rabbit IgG followed by Alexa Fluor 647-anti-rabbit IgG.
Reduced (R) and non-reduced (NR) SDS-PAGE analysis of purified lots of recombinant anti-human CD112R antibodies.
112R027 Image 05
Recombinant Anti-human CD112R (PVRIG) Antibody
CD112R (also known as PVRIG) is a coinhibitory receptor found on the surface of T cells. The high-affinity ligand for CD112R is CD112 which is expressed on antigen-presenting cells. This recombinant CD112R antibody is available in 3 formats for added flexibility.
Catalog Items:
- #201301 – Rabbit IgG, kappa – 50 µg – $99
- #201302 – Mouse IgG2a, kappa NullFc™ – 50 µg – $99
- #201303 – Biotinylated Rabbit Fab – 50 µg – $129
$99.00 – $129.00
Specifications
| Clone | 112R027 |
| Discovery Method | Rabbits were immunized with HEK293 cells expressing human CD112R, and antigen-positive B cells were single-cell sorted. The variable domain sequences were cloned, sequenced, and subcloned into expression vectors containing the various species and isotype constant domains |
| Available Formats | Rabbit IgG/kappa; Mouse IgG2a/kappa with NullFc™ (compatible with traditional anti-mouse secondary reagents); Biotinylated rabbit Fab with a single defined biotin and His-tag at the C-terminus of the heavy chain |
| Host Species | Rabbit |
| Immunogen | Human CD112R (accession BC107873) recombinantly expressed on HEK293 cells |
| Verified Reactivity | Human, cynomolgus monkey (Macaca fascicularis) |
| Verified Applications | Flow Cytometry |
| Concentration | 0.5 mg/ml |
| Production | Transient transfection expression system in mammalian HEK293 cells |
| Purification | Protein A affinity chromatography (MAb), Immobilized metal ion chromatography (Fab) |
| Formulation | PBS pH 7.2 with 0.08% sodium azide |
| Storage | 4° C for up to 3 months, for longer storage aliquot and store at -20° C |
| Target | CD112R |
| Alternate Names | PVRIG, C7orf15 |
| Target Description | CD112R is a single transmembrane domain protein that is a cell surface receptor for CD112 (Nectin-2). CD112R is preferentially expressed on T cells. Binding to CD112 results in the inhibition of T cell proliferation, thus functioning as a checkpoint receptor by suppressing TCR-mediated signaling. |
| Uniprot | Q6DKI7 |
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